De novo mutation rate estimation in wolves of known pedigree.

摘要: Knowledge of mutation rates is crucial for calibrating population genetics models demographic history in units years. However, remain challenging to estimate because the need identify extremely rare events. We estimated nuclear rate wolves by identifying de novo mutations a pedigree seven wolves. Putative were discovered whole-genome sequencing and verified Sanger parents offspring. Using stringent filters an false negative remaining observable genome, we obtain ∼4.5 x 10-9 per base pair generation provide conservative bounds from 2.6 7.1 10-9. Although our consistent with recent estimates ancient DNA (4.0 3.0-4.5 10-9), it implies wider possible range. also examined consequences accompanying interval dating several critical events canid history. For example, applying full range coalescent dog wolf wide set divergence times between ancestral populations dogs extant Eurasian (16,000 - 64,000 years ago) although point indicates date 25,000 33,000 ago. Aside one study mice, ours provides only direct mammalian outside primates, likely be vital future investigations evolution.