作者: Guangkun Yin , Hongmei Sun , Xia Xin , Guozheng Qin , Zheng Liang
DOI: 10.1093/PCP/PCP074
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摘要: The development of mitochondria during seed germination is essential for plant growth. However, the developmental process still poorly understood. Temperature plays a key role in soybean germination, and this study we characterized mitochondrial ultrastructure proteome after imbibition at 22, 10 4 degrees C 24 h. from axis can be divided into light heavy by Percoll density gradient centrifugation. axes imbibed mainly contained mitochondria, which had lower levels specific enzymes oxidative phosphorylation activity. In contrast, 22 exhibited higher metabolism. Electron microscopy revealed that developed internal membrane system with few cristae, while more normally. Furthermore, compared proteomes different temperatures. differentially expressed proteins were identified using ESI-Q-TOF-MS/MS (electrospray ionization quadrupole time-of-flight tandem mass spectrometry). Proteins involved metabolites including malate dehydrogenase (tricarboxylic acid cycle enzyme), putative ATP synthase subunit (oxidative complex subunits), chaperonin-60 (heat shock protein), arginase (urea enzyme) elongation factor Tu (mitochondrial genome transcript identified. reduced expression these might not support normal We conclude chilling causes damage both ultrastructural metabolic levels.