Reduced mouse fibroblast cell growth by increased hydrophilicity of microbial polyhydroxyalkanoates via hyaluronan coating.
作者： Ya-Wu Wang , Qiong Wu , Guo-Qiang Chen , None
DOI: 10.1016/S0142-9612(03)00356-9
关键词:
摘要: The mouse fibroblast cell line L929 was inoculated on 3D scaffolds of microbial polyesters, namely polyhydroxybutyrate (PHB) and poly(hydroxybutyrate-co-hydroxyhexanoate) (PHBHHx) to evaluate their in vitro biocompatibility. It found that both polyhydroxyalkanoates (PHA) subjected lipase treatment hyaluronan (HA) coating decreased the contact angle water material surface approximately 30%, meaning an increased hydrophilicity PHA surface. At same time, HA smoothened After or coating, ratio hydrophilic groups including hydroxyl carboxyl carbonyl 1:1 2:1. Cells grown treated with were 4 x 10(5)/ml, twice number control. However, coated observed a 40% decrease growth compared reduced attachment proliferation although materials had hydrophilicity. In comparison, promoted did not lead better coating. appeared appropriate combination hydrophobicity important for biocompatibility PHBHHx, especially cells this material. This may have instructive significance biomaterial selection design.
elsevier.com
sci-hub.se 参考文章(25)
David Byrom, Biomaterials: Novel Materials from Biological Sources ,(1991)
W. Norde, The Behavior of Proteins at Interfaces, with Special Attention to the Role of the Structure Stability of the Protein Molecule Clinical Materials. ,vol. 11, pp. 85- 91 ,(1992) , 10.1016/0267-6605(92)90032-O
Simon F Williams, David P Martin, Daniel M Horowitz, Oliver P Peoples, PHA applications: addressing the price performance issue: I. Tissue engineering International Journal of Biological Macromolecules. ,vol. 25, pp. 111- 121 ,(1999) , 10.1016/S0141-8130(99)00022-7
M. Abercrombie, Joan E.M. Heaysman, Susan M. Pegrum, The locomotion of fibroblasts in culture. IV. Electron microscopy of the leading lamella. Experimental Cell Research. ,vol. 67, pp. 359- 367 ,(1971) , 10.1016/0014-4827(71)90420-4
A J Anderson, E A Dawes, Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates. Microbiological Research. ,vol. 54, pp. 450- 472 ,(1990) , 10.1128/MR.54.4.450-472.1990
M. Abercrombie, E.J. Ambrose, Interference microscope studies of cell contacts in tissue culture Experimental Cell Research. ,vol. 15, pp. 332- 345 ,(1958) , 10.1016/0014-4827(58)90034-X
Jianqing Zhao, Georges Geuskens, Surface modification of polymers VI. Thermal and radiochemical grafting of acrylamide on polyethylene and polystyrene European Polymer Journal. ,vol. 35, pp. 2115- 2123 ,(1999) , 10.1016/S0014-3057(99)00026-9
Barbara D Boyan, Thomas W Hummert, David D Dean, Zvi Schwartz, Role of material surfaces in regulating bone and cartilage cell response Biomaterials. ,vol. 17, pp. 137- 146 ,(1996) , 10.1016/0142-9612(96)85758-9
J KNOWLES, G HASTINGS, H OHTA, S NIWA, N BOEREE, Development of a degradable composite for orthopaedic use: in vivo biomechanical and histological evaluation of two bioactive degradable composites based on the polyhydroxybutyrate polymer. Biomaterials. ,vol. 13, pp. 491- 496 ,(1992) , 10.1016/0142-9612(92)90099-A
D.J. Chauvel-Lebret, P. Pellen-Mussi, P. Auroy, M. Bonnaure-Mallet, Evaluation of the in vitro biocompatibility of various elastomers Biomaterials. ,vol. 20, pp. 291- 299 ,(1999) , 10.1016/S0142-9612(98)00181-1