Novel iso-branched ether lipids as specific markers of developmental sporulation in the myxobacterium Myxococcus xanthus.

摘要: Iso-fatty acids (FAs) are the dominant FA family in all myxobacteria analyzed. Furthermore, it was postulated that iso-FAs or compounds derived thereof involved fruiting body formation Myxococcus xanthus, since mutants with a reduced level of iso-FA due to precursor isovaleryl-CoA, delayed aggregation and produce only few myxospores. To elucidate function their corresponding lipids we have analyzed developmental phenotype having different levels resulting clear correlation between amount delay reduction spore yield. Addition either isovalerate 13-methyltetradecanoic acid resulted restoration wild-type profile normal development. Detailed analysis fatty (FA) during xanthus revealed specific accumulation 13-methyltetradecanal 1-O-13-methyltetradecylglycerol which were produced specifically myxospores from 1-O-(13-methyl-1-Z-tetradecenyl)-2-O-(13-methyltetradecanoyl)-glycero-3-phosphatidylethanolamine (VEPE) 1,2-di-(13-methyltetradecanoyl)-3-(13-methyltetradecyl)glycerol (TG-1), respectively. The structures these unusual ether been determined by spectrometric methods synthesis (for TG-1). Analysis several blocked at stages development indicated biosynthesis TG-1 is developmentally regulated VEPE might be an intermediate biosynthesis. Finally, addition isovaleryl-CoA could restore sporulation emphasizing important role iso-branched for myxobacterial