Differential effect of keratinocyte growth factor (KGF) on aromatase activity in cultured canine prostatic epithelial cells
作者： H. Canatan , F. Shidaifat , S. K. Kulp , Y. Zhang , W. Y. Chang
DOI: 10.1080/07435809709031860
关键词:
摘要: We have recently cloned the mRNA encoding KGF from canine prostate and produced recombinant (rcKGF) which specifically acts on cultured prostatic epithelial cells (CCPECs) possess receptors (Canatan et al., 1996; DNA Cell Biol. 15:247). In present study, effect of rcKGF aromatase activity in CCPECs young (6-month-old) mature (3-year-old) dogs was examined. Release 3H2O labeled substrate used as indicator activity. were pulsed with [1-B-3H]-androstenedione (1 μCi/ml, 6 hr). The amounts released into culture medium measured (dpm) total cellular proteins determined. Aromatase expressed dpm/mg protein (mean ± SEM). basal level approximately 4 times higher (p<0.05) than that dogs. increased a dose-dependent manner upon treatment rcKGF. Interestingl...
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