Cell Cycle Regulation of Human CDC6 Protein INTRACELLULAR LOCALIZATION, INTERACTION WITH THE HUMAN MCM COMPLEX, AND CDC2 KINASE-MEDIATED HYPERPHOSPHORYLATION
作者： Masatoshi Fujita , Chieko Yamada , Hidemasa Goto , Naoaki Yokoyama , Kiyotaka Kuzushima
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摘要: The binding of mammalian MCM complexes to chromatin is cell cycle-regulated and under CDC2 kinase negative control. Here, we investigated the properties CDC6 protein, a candidate regulator MCM. levels were relatively constant during HeLa cycle. In asynchronous cells, was mainly detected in nuclei with immunostaining, but some not extractable nonionic detergent. contrast chromatin-bound MCM, this fraction resistant DNase I treatment, suggesting that it binds detergent- nuclease-resistant nuclear structure. S phase became detectable cytoplasm immunostaining; however, level bound unchanged. G2/M still maintained, which hyperphosphorylated by kinase. These data suggest protein associated specific structure throughout cycle major sites on differ between CDC6. However, co-immunoprecipitation assays chemical cross-linking indicated small part present close
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sci-hub.se 参考文章(48)
M. Coué, S. E. Kearsey, M. Méchali, Chromotin binding, nuclear localization and phosphorylation of Xenopus cdc21 are cell-cycle dependent and associated with the control of initiation of DNA replication. The EMBO Journal. ,vol. 15, pp. 1085- 1097 ,(1996) , 10.1002/J.1460-2075.1996.TB00446.X
S. Piatti, C. Lengauer, K. Nasmyth, Cdc6 is an unstable protein whose de novo synthesis in G1 is important for the onset of S phase and for preventing a 'reductional' anaphase in the budding yeast Saccharomyces cerevisiae. The EMBO Journal. ,vol. 14, pp. 3788- 3799 ,(1995) , 10.1002/J.1460-2075.1995.TB00048.X
Masatoshi Fujita, Tohru Kiyono, Yasuyuki Hayashi, Masahide Ishibashi, hCDC47, a Human Member of the MCM Family DISSOCIATION OF THE NUCLEUS-BOUND FORM DURING S PHASE Journal of Biological Chemistry. ,vol. 271, pp. 4349- 4354 ,(1996) , 10.1074/JBC.271.8.4349
M Kusubata, T Tokui, Y Matsuoka, E Okumura, K Tachibana, S Hisanaga, T Kishimoto, H Yasuda, M Kamijo, Y Ohba, p13suc1 suppresses the catalytic function of p34cdc2 kinase for intermediate filament proteins, in vitro. Journal of Biological Chemistry. ,vol. 267, pp. 20937- 20942 ,(1992) , 10.1016/S0021-9258(19)36779-1
John F.X. Diffley, Julie H. Cocker, Simon J. Dowell, Adele Rowley, Two steps in the assembly of complexes at yeast replication origins in vivo Cell. ,vol. 78, pp. 303- 316 ,(1994) , 10.1016/0092-8674(94)90299-2
S. Donovan, J. Harwood, L. S. Drury, J. F. X. Diffley, Cdc6p-dependent loading of Mcm proteins onto pre-replicative chromatin in budding yeast Proceedings of the National Academy of Sciences of the United States of America. ,vol. 94, pp. 5611- 5616 ,(1997) , 10.1073/PNAS.94.11.5611
James P. J. Chong, Hiro M. Mahbubani, Chong-Yee Khoo, J. Julian Blow, Purification of an MCM-containing complex as a component of the DNA replication licensing system Nature. ,vol. 375, pp. 418- 421 ,(1995) , 10.1038/375418A0
Jacqueline Hayles, Daniel Fisher, Alison Woollard, Paul Nurse, Temporal order of S phase and mitosis in fission yeast is determined by the state of the p34cdc2-mitotic B cyclin complex Cell. ,vol. 78, pp. 813- 822 ,(1994) , 10.1016/S0092-8674(94)90542-8
Kai Stoeber, Anthony D Mills, Yumiko Kubota, Torsten Krude, Piotr Romanowski, Kathrin Marheineke, Ronald A Laskey, Gareth H Williams, Cdc6 protein causes premature entry into S phase in a mammalian cell‐free system The EMBO Journal. ,vol. 17, pp. 7219- 7229 ,(1998) , 10.1093/EMBOJ/17.24.7219
Chieko Mineo, Yasufumi Murakami, Yukio Ishimi, Fumio Hanaoka, Masa-Atsu Yamada, Isolation and analysis of a mammalian temperature-sensitive mutant defective in G2 functions. Experimental Cell Research. ,vol. 167, pp. 53- 62 ,(1986) , 10.1016/0014-4827(86)90203-X