New Properties of Drosophila scs and scs’ Insulators

摘要: Insulators are defined as a class of regulatory elements that delimit independent transcriptional domains within eukaryotic genomes. The first insulators to be identified were scs and scs', which flank the domain including two heat shock 70 genes. Zw5 BEAF bind respectively, responsible for interaction between these insulators. Using regions yellow white reporter genes, we have found scs' improves enhancer-blocking activity weak insulator. sequences include promoters genes strongly active in S2 cells but not eyes, has been extensively examined. Only promoter Cad87A gene located at end insulator drives expression enhancer can slightly stimulate this promoter. contains polyadenylation signals may important preventing transcription through As shown previously, insulate transgene from enhancing effects surrounding genome, phenomenon known chromosomal position effect (CPE). After analyzing many transgenic lines, concluded transgenes carrying rarely inserted into genomic eyes.