Effect of the pH in the enrichment of X or Y sex chromosome-bearing sperm in bovine.

作者: Nidhi P. Raval , Tejas M. Shah , Linz-Buoy George , Chaitanya G. Joshi

DOI: 10.14202/VETWORLD.2019.1299-1303

关键词:

摘要: Background and Aim Studies have shown that the pH of vagina during course fertilization may influence migration X- Y-bearing spermatozoa thus leading to skewness in sex offspring. Hence, this study was carried out check effect enrichment X or Y chromosome-bearing sperm bovine (Bos indicus). Materials Methods To bovine, we used buffers various ranging from 5.5 9.0 for swim-up procedure sample collected upper bottom fraction same buffer checked abundance by droplet digital polymerase chain reaction using Y-chromosome-specific DNA probe. Results The not differed significantly either collected. Conclusion Thus, it appears be unlikely an immediate impact on can a solitary offspring bovine.

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