Significant enrichment of Y-bearing chromosome human spermatozoa using a modified centrifugation technique.

摘要: Summary The effective separation of X- and Y-bearing chromosome spermatozoa has been a topic major attraction to number scientific disciplines. Approaches have typically based upon either the kinetic or physical characteristics spermatozoa. Much information available date suggested conflicting evidence between different approaches lack repeatability, while other robust reproducible techniques require expensive equipment are being questioned with relation their safety in clinical applications. This study describes safe efficient method for successful enrichment cells from X counterparts human male using simple approach on centrifugation. Five donor candidates normal semen profiles proven fertility were recruited. In total, 20 specimens processed conventional swim-up. During each attempt, half swim-up product was subjected technique served as control. Parameters important successfully skewing sex ratios included relative centrifugal force, duration centrifugation rounds. Assessment samples following effected by three-colour-labelled fluorescent situ hybridization. More than 1000 specimen assessed presence an Y chromosome. The produced significantly higher (p < 0.001) overall frequency 85.5% experimental group (3606 X-bearing/21 209 Y-bearing) compared 50.1% control (11 801 X-bearing/11 269 Y-bearing), where there no statistically significant difference Y-chromosome-bearing conclusion, can be reproducibly achieved use followed meticulous protocol.