Dendritic and Axonal Targeting of Type 5 Metabotropic Glutamate Receptor Is Regulated by Homer1 Proteins and Neuronal Excitation

作者: Fabrice Ango , Jean-Philippe Pin , Jian Chen Tu , Bo Xiao , Paul F. Worley

DOI: 10.1523/JNEUROSCI.20-23-08710.2000

关键词:

摘要: The physiological actions of neurotransmitter receptors are intimately linked to their proper neuronal compartment localization. Here we studied the effect metabotropic glutamate receptor (mGluR)-interacting proteins, Homer1a, b, and c, in targeting mGluR5 neurons. We found that was exclusively localized cell bodies when transfected alone cultured cerebellar granule cells. In contrast, also dendrites coexpressed with Homer1b or Homer1c, both axons cotransfected Homer1a. dendrites, Homer1b/c formed clusters colocalized synaptic marker synaptophysin. Interestingly alone, Homer proteins were translocated neurites but did not form such clusters. Depolarization neurons a mixture ionotropic agonists, NMDA kainate, potassium channel blockers, tetraethylammonium 4-aminopyridine, induced transient expression endogenous Homer1a persistent neuritic localization even long after degradation These results suggest Homer1a/b/c involved dendritic sites and/or this can be regulated by activity. Because activity-dependent long-lasting, axonal protein is likely play an important role plasticity.

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