Detection of Fasciola hepatica and Fasciola gigantica common and uncommon antigens, using rabbit hyper immune serum raised against their excretory–secretory and somatic antigens
作者： S. Abdolahi Khabisi , B. Sarkari
DOI: 10.1007/S12639-015-0726-5
关键词:
摘要: Fasciolosis is an important neglected helminth disease caused by two liver flukes, Fasciola hepatica and gigantica. The species of are usually different in their morphological molecular features. They have also common uncommon antigens both somatic excretory secretory metabolites. In this study, we compared excretory–secretory (ES) F. gigantica, using rabbit hyper immune serum raised against these antigens. Adult worms were collected from bile ducts infected animals the fluke was confirmed RFLP-PCR. ES prepared. Rabbits subcutaneously immunized with either or to produce antibodies SDS-PAGE pattern gigantica similar them revealed 30 protein bands, ranging 18 180 kDa. contrast, antigen different. While bands weight 18, 27, 29, 48, 62 kDa species, 19, 45, 55 58 only noticed antigen. Rabbit polyclonal antibodies, antigen, reacted main five 25, 67 three 27 72 Thus, 29 may serve as immunodominant antigens, which might be considered for serodiagnosis fasciolosis. Moreover, suitable candidate development serological assays diagnosis
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