The Genome of the Nucleopolyhedrosis-Causing Virus from Tipula oleracea Sheds New Light on the Nudiviridae Family

摘要: ABSTRACT A large double-stranded DNA (dsDNA) virus that produces occlusion bodies, typical of baculoviruses, has been described to infect crane fly larvae the genus Tipula (Diptera, Tipulidae). Because a lack genomic data, this remained unclassified. Electron microscopy an archival isolated from oleracea, T. oleracea nudivirus (ToNV), showed irregularly shaped bodies measuring 2 5 μm in length and middiameter, filled with rod-shape virions containing single nucleocapsids within bilayer envelope. Whole-genome amplification Roche 454 sequencing revealed complete circular genome sequence 145.7 kb, five direct repeat regions. We predicted 131 open reading frames, including homolog polyhedrin gene encoding major body protein paludosa nucleopolyhedrovirus (NPV). BLAST searches demonstrated ToNV had 21 37 baculovirus core genes but shared 52 nudiviruses (NVs). Phylogenomic analyses indicated clearly belongs Nudiviridae family should probably be assigned new genus. Among nudiviruses, was most closely related Penaeus monodon NV Heliothis zea clade distantly Drosophila innubia NV, other infecting Diptera. Lastly, found nuvidirus ancestor bracoviruses. This also reflected terms content, as only known exogenous harboring homologs Cc50C22.6 27b ( Cc50C22.7 ) nudiviral cluster involved bracovirus particle production. IMPORTANCE The is arthropod dsDNA viruses which striking cases endogenization have reported (i.e., symbiotic bracoviruses deriving endogenous brown planthopper). Although relatively little about diversity nudiviruses. Here, we characterized, morphologically genetically, sample particularity forming bodies. Comparative phylogenomic date closest relative Moreover, revised homology relationships identified set 32 for Nudiviridae, were genes. These findings provide important insights into evolutionary history viruses.