Modulation of CD4+ T cell responses following splenectomy in hepatitis C virus‐related liver cirrhosis

摘要: Dysfunction of T cells is a common feature in chronic persistent viral infections, including hepatitis C virus (HCV), and although hepatic peripheral have been studied extensively HCV hepatitis, the role splenic cell responses such patients poorly defined. This an important issue, as thrombocytopenia complication HCV-related liver cirrhosis (LC), due to platelet sequestration bone marrow suppression; splenectomy has proposed treat patients. Herein, we blood mononuclear (PBMC) lymphoid subpopulations from total 22 patients, 15 with LC marked treated splenectomy, seven controls. CD4(+) spleen were isolated phenotype function evaluated. Splenic expressed molecules associated inhibitory signalling, increased frequency negative markers cytotoxic lymphocyte antigen-4 (CTLA-4) programmed death 1 (PD-1) decreased production cytokines. Patients manifest higher levels regulatory PD-L1- PD-L2-expressing than Blocking PD-1/PD-1 ligand interaction reconstituted proliferative cytokine (SMC) LC. Splenectomy was followed by increase ratio interferon (IFN)-γ interleukin (IL)-10 reduction PD-1-expressing blood. Our data suggest that tolerance promoted via up-regulated expression PD-1 ligands.