Characteristics of splenic CD8+ T cell exhaustion in patients with hepatitis C

摘要: There is increasing interest in the role of T cell exhaustion and it well known that natural history chronic hepatitis C virus infection (HCV) modulated by CD8(+) immunobiology. are many pathways alter presence exhaustive cells and, particular, they functionally impaired inhibitory receptors, such as programmed death-1 (PD-1) immunoglobulin mucin domain-containing protein 3 (Tim-3). We obtained spleen, liver peripheral blood (before after splenectomy) lymphoid from 25 patients with HCV-related cirrhosis undergoing transplantation for end-stage disease or splenectomy portal hypertension. In all samples we performed an extensive phenotypic study markers [PD-1, Tim-3, interferon (IFN)-γ) their ligands (PD-L1, PD-L2, galectin-9] subpopulations (both total HCV-specific) antigen-presenting (APC; monocytes dendritic cells). HCV-specific demonstrated enhanced exhaustion, predominantly effector memory subpopulation. Similarly, splenic APC over-expressed receptor when compared to blood. Finally, were before splenectomy, reduced APC. Our data suggest spleen manifest a significantly higher may thus contribute failure control HCV. Counteracting this process inducing effective immune response