The 1996 Moyer Award: Effects of Endotoxin on the Th1/th2 Response in Humans

摘要: Monocyte/T-cell interactions play a critical role in the systemic response to infection. Distinct patterns of cytokines are produced by two different types T-helper cells (Th). Thl secrete interleukin-2 (IL-2) and interferon-γ (IPN-γ), whereas Th2 produce IL-4, IL-5, IL-6, IL-10, IL-13. In volunteers endotoxin administration initiates many features gram-negative sepsis including cytokine release, but (i.e., Th1/Th2 patterns) have not yet been studied. this institutional review board-approved study we investigated effect an intravenous bolus from Escherichia coli (4 ng/kg body weight) on four female male (mean age 27,1 ± 0.8 years). Plasma levels for IL-2, IL-12, IFN-γ heart rate, mean arterial pressure, temperature, white blood cell, differential count were determined before hourly 5 hours after administration. All had tachycardia, decreased fever, leukocytosis. IL-10 was significantly (p < 0.05) elevated (9.4 3.9 pg/ml vs 60.9 19.3 pg/ml) 3 administered, IL-2 (69 26 U/ml 30.6 14.9 U/ml). IL-4 detectable plasma. No changes seen plasma IL-12. Systemic responses did correlate with levels. Cytokine found suggest that low-dose T-cell immune is shifted towards cell type response. This early shift may contribute depressed cell-mediated associated sepsis.