The role of the DNA mismatch repair system in the cytotoxicity of the topoisomerase inhibitors camptothecin and etoposide to human colorectal cancer cells.

作者: Françoise Praz , Sandrine Jacob , Mélanie Aguado , David Fallik

DOI:

关键词:

摘要: The DNA mismatch repair (MMR) system is involved in the correction of base/base mismatches and insertion/deletion loops arising during replication. In addition, some MMR components participate recombination double-strand break as well cell cycle regulation apoptosis. inactivation genes, usually hMSH2 or hMLH1, associated with human colorectal cancers responsible for characteristic microsatellite instability (MSI)+ phenotype these tumors. Because assumed to modulate cytotoxicity various chemotherapeutic agents that act upon DNA, our objectives have been define its possible involvement topoisomerase inhibitors. We shown cancer lines defective exhibit an increased sensitivity both camptothecin, a I inhibitor, etoposide, II inhibitor. Sensitivity drugs cannot be predicted by measuring endogenous levels II. Our results also indicate neither p53 status, nor alterations correlate cells On other hand, data showing resistance can achieved functional complementation hMLH1 hMLH1-defective line allowed us establish critical determinant chemosensitivity. Interestingly, observations provide rationale better responsiveness MSI+ tumors CPT-11, camptothecin derivative, which we observed patients metastatic cancers.

参考文章(65)
Frédéric Pâques, James E. Haber, Multiple Pathways of Recombination Induced by Double-Strand Breaks in Saccharomyces cerevisiae Microbiology and Molecular Biology Reviews. ,vol. 63, pp. 349- 404 ,(1999) , 10.1128/MMBR.63.2.349-404.1999
Margaret Ashcroft, Yoichi Taya, Karen H. Vousden, Stress signals utilize multiple pathways to stabilize p53. Molecular and Cellular Biology. ,vol. 20, pp. 3224- 3233 ,(2000) , 10.1128/MCB.20.9.3224-3233.2000
Ryuichi Mibu, Eiitsu Nakajima, Kenji Shimizu, Akinori Kimura, Nagahide Matsubara, Noriaki Tanaka, Takashi Shimada, Hideo Orimo, Masahiko Ikeda, Hiroki Moriyama, Close Correlation between Mutations of E2F4 and hMSH3 Genes in Colorectal Cancers with Microsatellite Instability Cancer Research. ,vol. 58, pp. 594- 598 ,(1998)
C Richard Boland, Stephen N Thibodeau, Stanley R Hamilton, David Sidransky, James R Eshleman, Randall W Burt, Stephen J Meltzer, Miguel A Rodriguez-Bigas, Riccardo Fodde, G Nadia Ranzani, Sudhir Srivastava, None, A National Cancer Institute Workshop on Microsatellite Instability for Cancer Detection and Familial Predisposition: Development of International Criteria for the Determination of Microsatellite Instability in Colorectal Cancer Cancer Research. ,vol. 58, pp. 5248- 5257 ,(1998)
Maria Kraakman-van der Zwet, Wilhelmina J.I Overkamp, Anna A Friedl, Binie Klein, Gerald W.C.T Verhaegh, Nicolaas G.J Jaspers, Alina T Midro, Friederike Eckardt-Schupp, Paul H.M Lohman, Małgorzata Z Zdzienicka, Immortalization and characterization of Nijmegen Breakage syndrome fibroblasts. Mutation Research-dna Repair. ,vol. 434, pp. 17- 27 ,(1999) , 10.1016/S0921-8777(99)00009-9
Yves Pommier, Kurt W. Kohn, Joany Jackman, Tsunehiro Shimizu, François Goldwasser, Yuko Hoki, Patrick M. O'Connor, Correlations between S and G2 arrest and the cytotoxicity of camptothecin in human colon carcinoma cells. Cancer Research. ,vol. 56, pp. 4430- 4437 ,(1996)
E Georges, R K Prichard, B Nare, Characterization of rhodamine 123 binding to P-glycoprotein in human multidrug-resistant cells. Molecular Pharmacology. ,vol. 45, pp. 1145- 1152 ,(1994)
P Branch, M Masson, G Aquilina, M Bignami, P Karran, Spontaneous development of drug resistance: mismatch repair and p53 defects in resistance to cisplatin in human tumor cells. Oncogene. ,vol. 19, pp. 3138- 3145 ,(2000) , 10.1038/SJ.ONC.1203668
Emma L Arriola, Ana Rodriguez Lopez, Christine M Chresta, Differential regulation of p21waf-1/cip-1 and Mdm2 by etoposide: etoposide inhibits the p53-Mdm2 autoregulatory feedback loop. Oncogene. ,vol. 18, pp. 1081- 1091 ,(1999) , 10.1038/SJ.ONC.1202391
Valérie Boige, Francesco Borrini, Jean-Christophe Sabourin, Francoise Praz, Sandrine Jacob, Michel Ducreux, David Fallik, [Response of metastatic colorectal cancers to treatment with CPT11 (irinotecan): implications of the mismatched base repair system]. Gastroenterologie Clinique Et Biologique. ,vol. 24, pp. 917- 922 ,(2000)