Chemistry of a Unique Polyketide-like Synthase
作者： Stephanie W. Chun , Meagan E. Hinze , Meredith A. Skiba , Alison R. H. Narayan
DOI: 10.1021/JACS.7B13297
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摘要: Like many complex natural products, the intricate architecture of saxitoxin (STX) has hindered full exploration this scaffold’s utility as a tool for studying voltage-gated sodium ion channels and pharmaceutical agent. Established chemical strategies can provide access to product; however, chemoenzymatic route that could expedited related compounds not been devised. The first step toward realizing approach class molecules is elucidation biosynthetic pathway. To date, biochemical link between STX its putative enzymes demonstrated. Herein, we report characterization any enzyme involved in biosynthesis. Specifically, functions polyketide-like synthase, SxtA, from cyanobacteria Cylindrospermopsis raciborskii T3 are elucidated. This unique megasynthase comprised four domains: methyltransferase (MT), GCN5-related N-...
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