Highly Site Specific, Protease Cleavable, Hydrophobic Peptide–Polymer Nanoparticles
作者： Matthias Maier , Niklas Kotman , Cornelius Friedrichs , Julien Andrieu , Manfred Wagner
DOI: 10.1021/MA201149B
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摘要: Here we present the synthesis of site specific, protease (trypsin) cleavable, hydrophobic peptide–polymer conjugate nanoparticles in inverse miniemulsion. Different peptide sequences were synthesized by microwave-assisted solid-phase and characterized HPLC, NMR, MALDI-TOF MS. The peptides used to cross-link a functional polymer with standard peptide-coupling chemistry miniemulsion, thus generating desired nanoparticulate morphology. By these means, well-defined size range 200–300 nm obtained. After transferring particles into aqueous medium, they photon cross-correlation spectroscopy (PCCS) scanning electron microscopy (SEM). removal solvent, degree cross-linking was determined 15N solid-state NMR. Integration fluorophore/quencher system, based on fluorescence resonance energy transfer (FRET) sequences, enabled monitoring specific recognition ...
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