Application of a bonded-phase extraction column for rapid sample preparation of flecainide from human plasma for high-performance liquid chromatographic analysis--fluorescence or ultraviolet detection.

摘要: A simple, rapid, selective, and sensitive procedure for the monitoring of flecainide levels in human plasma is described. The drug internal standard--a positional isomer flecainide--are separated from by use a disposable extraction column packed with reversed-phase sorbent. standard which are selectively retained on eluted. An aliquot injected onto mu-Bondapak phenyl eluted mixture acetonitrile 0.06% phosphoric acid (40:60, vol/vol) at flow rate 2 ml/min. measured fluorescence detector excitation emission wavelengths 300 370 nm, respectively. linear range 3-2,000 ng/ml, sensitivity limit 3 ng/ml 1 ml plasma. within-day coefficient variation was 1.1-4.6% concentrations ranging to 1,600 ng/ml. Many drugs given concomitantly acetate do not interfere assay. method compares favorably well-documented gas-liquid chromatographic suitable level patient management as well pharmacokinetic studies. Similar results were obtained using an alternate detection (ultraviolet 298 nm).