Liquid chromatography and fluorescence polarization immunoassay methods compared for measuring flecainide acetate in serum.
作者： R J Straka , T J Hoon , R L Lalonde , J A Pieper , M B Bottorff
DOI: 10.1093/CLINCHEM/33.10.1898
关键词: Statistical difference 、 Fluorescence polarization immunoassay 、 Chromatography 、 Chemistry 、 Therapeutic drug monitoring 、 Flecainide Acetate 、 High-performance liquid chromatography 、 Serum samples
摘要: We analyzed 99 patients' serum samples for concentrations of a new antiarrhythmic agent, flecainide acetate, by fluorescence polarization immunoassay (FPIA) and "high-performance" liquid chromatography (HPLC). Within-day between-day coefficients variation at in the low high ends therapeutic range were less than 7% HPLC 9% FPIA. There was no statistical difference mean (+/- SD) clinical measured two methods (607 +/- 334 micrograms/L HPLC, 602 344 FPIA), but results each differed 0.13%. FPIA measurements correlated significantly (r = 0.98, P 0.05), linearly related (slope 0.970, intercept 13 micrograms/L) as assessed orthogonal regression. Both assay produced similar concentration sufficiently accurate precise to be used drug monitoring.
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