ADNP/ADNP2 expression in oligodendrocytes: implication for myelin-related neurodevelopment.

摘要: Oligodendrocytes, the myelin-forming cells of central nervous system, play important roles in brain development and maintenance. Activity-dependent neuroprotective protein (ADNP), an early marker essential for formation, interacts with microtubule end-binding proteins (EB1, EB2, EB3). EB1 EB3 are highly expressed neurons (axons dendritic spines, respectively) enhancement neurite outgrowth is attenuated by EB2. ADNP/EB presence oligodendrocytes has not been studied so far. Here, we measured messenger RNA (mRNA) levels ADNP EB1-EB3 rat during culture maturation brains (1, 35, 75 days) comparison astrocytes, dorsal root ganglion (DRG) neurons, oligodendroglia cell lines (OLN-93 line, expressing microtubule-associated (MAP) tau, OLN-93 stably transfected to express various forms tau). Results showed that all transcripts were oligodendrocytes. EB2 mRNA transcript content peaked at time oligodendrocyte (5 days vitro) was highest newborn compared mature brains. ADNP2 (the only family member ADNP), EB1, although lower quantities, essentially paralleled expression patterns, respectively. mRNA, peaking upon maturation, apparent second peak (10 days increased brain. DRG EB3, when precursors astrocytes but Mature ~30-40-fold more vs. ~4-7-fold ADNP. DRGs ~5-fold in-between (~20-fold) ratio. Only similar levels, contrasting (~10-30-fold EB1). Astrocytes than precursor (~2-fold) previously found be associated tau. Immortalized intermediate phenotype tau transfection reducing overall EB expression. In summary, ADNPs EBs suggesting impact on myelin formation health disease.