Application of Quantitative Immunogold Electron Microscopy to Determine the Distribution and Relative Expression of Homo- and Heteromeric Purinergic Adenosine A1 and P2Y Receptors

摘要: The idea that G-protein-coupled receptors (GPCRs) may generate or modify various functions as dimmers higher-order oligomers is now generally accepted. Significant numbers of GPCRs exit heteromeric assemblies (refered to hetero-oligomerization), generating novel for ligand binding and second messengers, in turn creating unique receptor trafficking systems pharmacological profiles (Angers et al., 2002, Bulenger 2005). This also true the purinergic family. Over recent years, we have explored many biochemical aspects this particular family via hetero-oligomerization between metabotropic (i.e. G protein-coupled) (particularly P1 P2), which agonists are metabolites playing important role signaling cascade.