Protective effects of aqueous and ethanol extracts of rosemary on H2O2-induced oxidative DNA damage in human lymphocytes by comet assay.
作者： Kamal Razavi-Azarkhiavi , Javad Behravan , Fatemeh Mosaffa , Samira Sehatbakhsh , Kobra Shirani
关键词:
摘要: BACKGROUND Rosemary (Rosmarinus officinalis) possesses various pharmacological properties such as antioxidant, anti-tumorigenesis and anti-mutagenesis activities. In this study, we investigated the possible protective effects of ethanol aqueous extracts rosemary on human lymphocyte DNA damage induced by H2O2. The extent lesions was measured using comet assay. METHODS Blood samples were taken from healthy volunteers lymphocytes isolated. then incubated in extract (0.05, 0.1, 0.5, 1 2.5 mg/mL) H2O2 (50, 100 200 mM). Lymphocytes also with a combination (100 mM) either or mg/mL both for 30 min at 4°C. RESULTS Our findings showed that treatment led to significant concentrate-dependent when compared respective controls (p<0.001). which initially occurred result µM (Percentage tail 55.1%) inhibited due doses tested (percentage 4.7% 4.03%). However, has no genotoxicity. CONCLUSIONS We suggest antioxidant constituents can prevent oxidative is its free radical scavenging activity.
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