Combined use of bone marrow-derived mesenchymal stromal cells (BM-MSCs) and platelet rich plasma (PRP) stimulates proliferation and differentiation of myoblasts in vitro: new therapeutic perspectives for skeletal muscle repair/regeneration.

摘要: Satellite cell-mediated skeletal muscle repair/regeneration is compromised in cases of extended damage. Bone marrow mesenchymal stromal cells (BM-MSCs) hold promise for healing but some criticisms hamper their clinical application, including the need to avoid animal serum contamination expansion and scarce survival after transplant. In this context, platelet-rich plasma (PRP) could offer advantages. Here, we compare effects PRP or standard culture media on C2C12 myoblast, satellite cell BM-MSC viability, survival, proliferation myogenic differentiation evaluate PRP/BM-MSC combination promoting differentiation. induced an increase mitochondrial activity Ki67 expression comparable even greater than that elicited by promoted AKT signaling activation myoblasts BM-MSCs Notch-1 pathway BM-MSCs. It stimulated MyoD, myogenin, α-sarcomeric actin MMP-2 activation. Notably, was more effective alone. We found influenced myoblast responses through a paracrine signaling, contributing shed light action mechanisms. Our results suggest represents good substitute manipulation vitro be beneficial towards transplanted vivo. Moreover, it might influence resident progenitors’ fate, thus favoring endogenous Finally, within limitations experimentation, study provides experimental background considering as potential therapeutic tool damage, combining tissue, while potentiating functionality.