Development and characterization of a continuous macrophage cell line, LRTM, derived from thymus of Labeo rohita (Hamilton 1822).

摘要: A long-term thymic macrophage cell line from the thymus explants of Labeo rohita designated as LRTM (L. macrophages) was established, which has been maintained in culture for more than 1 yr. This cells have subcultured 70 passages. The shape initially long and elongated; with subsequent passages, became short epithelial like. exhibited optimum growth L-15 containing 10% fetal bovine serum also Dulbecco’s modified Eagle’s medium at 37°C 5% CO2 showed 85+−% viability after 12 mo storage liquid nitrogen. In addition, nonspecific esterase surface expression Fc receptors immunoglobulin G classes I II major histocompatibility complex antigens. These observations confirmed that this had morphologic functional features a macrophage. phagocytic activity by engulfing yeast well fluorescent latex beads, demonstrated scanning electron microscopy Giemsa staining. cultured show rapid production reactive oxygen nitrogen intermediates following stimulation lipopolysaccharides phorbol miristate acetate (PMA). Mostly, all were alpha napthyl positive. After PMA lipopolysaccharide, fish macrophages produced intermediates. karyotype analysis these tetraploid 100 chromosomes each cell, indicating they are normal L. cells. Amplification, sequencing, alignment fragments two mitochondrial genes 12S rRNA rohu originated rohita. should be useful studying role differentiation maturation thymocytes can source macrophage-specific enzymes cytokines. will invaluable studies pathogen/macrophage interactions, mechanisms antimicrobial effector functions contribution to specific immune responses teleosts.