Cell and Tissue Culture

摘要: Publisher Summary The culturing, development, and monitoring requirements vary with the goals, cell type, species under investigation, much information can be obtained from protocols for developing specific types of fish cultures general functions in cultures. This chapter describes methods primary long-term hemopoietic fish. An active culture continuously produces blood cells a period up to six months. The influence various additions basic medium (for example, toxicants, growth factors, cytokines) provides about factors that perturb formation. measuring parameters useful both research aquatic toxicology are outlined chapter. These viability induction 7-ethoxyresorufin-o-deethylase (EROD) activity results assays measured rapidly inexpensively microwell multiwell fluorometric plate reader, improving utility many disciplines but especially toxicology. used is rainbow trout (Oncorhynchus mykiss) because it most commonly research.