Two factors secreted by the goldfish optic nerve induce retinal ganglion cells to regenerate axons in culture

摘要: Unlike mammals, lower vertebrates can regenerate an injured optic nerve and other pathways of the CNS throughout life. We report here that in dissociated cell culture, goldfish retinal ganglion cells their axons response to two factors derived from sheath nerve. Axogenesis factor 1 (AF-1) is a small peptide (700–900 Da) inactivated by treatment with proteinase K but heat stable. A second factor, AF-2, polypeptide ca 12 kDa. In absence these factors, remained viable serum-free, defined media for at least week showed little outgrowth, as visualized using vital dye 5,6-carboxyfluorescein diacetate (5,6- CFDA). The addition AF-1 induced up 25% culture extend processes > 75 microns length 6 d; AF-2 had lesser highly significant effect. To verify neurite outgrowth was per se, we applied lipophilic 4-Di-10- ASP tectum allowed it diffuse several days before culturing retina. far greater percentage containing axonal than observed overall population, indicating are selective targets factors. effects or were not secondary enhanced viability, since neither survival nor number remaining after d affected presence activity mimicked tested over broad concentration range, example, NGF, BDNF, NT-3, CNTF, taurine, retinoic acid, acidic basic fibroblast growth considerably higher CM homogenates, suggesting actively secreted; has similar intra- extracellularly. Insofar derive act upon cells, they likely be important inducing regeneration vivo.