One-step extraction of plant nucleic acids
作者： Jean-Pierre Laulhere , Claude Rozier
DOI: 10.1016/0304-4211(76)90053-5
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摘要: Abstract A very rapid method of extraction the nucleic acids is described. The presence 20 % ethanol in medium enhances solubility and reactivity reagents. deproteinizing agents are diethylpyrocarbonate (DEP), sodium lauryl sulfate (SLS) chloroform. Differential rRNA, DNA sRNA 3 M acetate used alone or associated with sepharose gel filtration for a complete separation between main groups acids. Yield quality shown by migration on electrophoresis filtration. rRNAs from ribonuclease-rich tissues (Marchantia) not degraded.
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