Anaphase onset does not require the microtubule-dependent depletion of kinetochore and centromere-binding proteins.

摘要: Spindle checkpoint proteins, such as Mad2 and BubR1, the motors dynein/dynactin CENP-E usually leave kinetochores prior to anaphase onset by microtubule-dependent mechanisms. Likewise, 'chromosome passenger proteins' including INCENP are depleted from centromeres after then move midzone complex, an event that is essential for cytokinesis. Here we test whether cell cycle changes occur at require or contribute depletion of kinetochore centromere proteins independent microtubules. This required development a novel non-antibody method induce precocious in vivo using bacterially expressed fragment spindle protein Mad1 capable activating APC/C, called GST-Mad1F10. By injecting PtK1 cells nocodazole with GST-Mad1F10 processing immunofluorescence microscopy sister chromatid separation found Mad2, cytoplasmic dynein, 3F3/2 phosphoepitope remain on kinetochores. Thus these (or phosphoepitope) not does produce their In contrast, both microtubules passenger' centromeres, chromosomes presence absence microtubules, but