Construction and characterization of a Salmonella typhi-based human immunodeficiency virus type 1 vector vaccine

摘要: Abstract Since the human immunodeficiency virus type 1 (HIV-1) is transmitted either parenterally or sexually, both systemic and mucosal immune responses might be required to provide protective immunity. One option express HIV proteins in attenuated Salmonella vectors that elicit compartments. The first step constructing such a strain was achieved by integrating gene expression cassette encoding recombinant HIV-1 gp120 (rgp120) into aroC locus of an vaccine S. typhi . This rgp120 utilizes strong constitutive promoter, P lpp/lacUV5 , produces 0.05–0.1% total bacterial cell protein. Immunoblot analysis shows strains containing integrated protein recognized anti-gp120 monoclonal antibodies (mAbs) appropriate size for nonglycosylated full-length (52 kDa). also demonstrates as monomers multimers found predominantly insoluble fraction bacteria. Antigen-capture ELISA, using specific continuous epitopes on gp120, revealed exposure these -expressed differs from baculovirus-expressed binds CD4. Epitopes conserved region (109–113) third conserved/fourth variable regions (376–380, 382–384, 395–400) are more “surface-exposed”, while one epitope (313–324) “buried” relative corresponding baculovirus expressed gp120. Antibodies recognizing discontinuous CD4 binding domain do not react with rgp120. significance observations development discussed.