Comparison of antigen presentation of influenza A nucleoprotein expressed in attenuated AroA- Salmonella typhimurium with that of live virus.

摘要: Rationally attenuated strains of Salmonella expressing foreign proteins represent a potentially important vaccine delivery system. The characteristics Ag presentation influenza nucleoprotein expressed in an AroA- strain typhimurium (SL3262-pNP-2) have therefore been compared with those soluble purified (NP) and infectious virus. This represents three distinct modes internalization the same protein into APC. Human monocytes monocytic leukemia cell line THP-1 infected SL3261-pNP-2 were found to present several different epitopes from NP human CD4+ class II-restricted T lymphocytes. these clones was enhanced by pretreatment cells IFN-gamma but not TNF-alpha. Bacterial phagocytosis increased after opsonization immune serum. Macrophages unable I-restricted cells. In contrast, live virus, although recognized NP-specific CTL, inefficiently SL3261-pNP-2, recombinant NP, synthetic peptide 206-229, inhibited chloroquine protease inhibitors pepstatin A leupeptin, suggesting that major route processing each case via exogenous pathway. recognition all systems also abrogated cycloheximide brefeldin treatment, indicating requirement for recently synthesized MHC II molecules whole corresponding peptide.