pfcrt Polymorphism and the spread of chloroquine resistance in Plasmodium falciparum populations across the Amazon Basin.
作者： Pedro Paulo Vieira , Marcelo Urbano Ferreira , Maria das Graças Alecrim , Wilson D. Alecrim , Luiz Hidelbrando P. da Silva
DOI: 10.1086/422006
关键词:
摘要: The widespread occurrence of drug-resistant malaria parasites in South America presents a formidable obstacle to disease control this region. To characterize parasite populations and the chloroquine-resistance profile Plasmodium falciparum Amazon Basin, we analyzed DNA segment pfcrt gene, spanning codons 72-76, genotyped 15 microsatellite (MS) markers 98 isolates from 6 areas Brazil, Peru, Colombia where is endemic. K76T mutation, which critical for chloroquine resistance, was found all isolates. Five haplotypes (S[tct]MNT, S[agt]MNT, CMNT, CMET, CIET) were observed, including 1 previously Asian/African MS genotyping showed relatively homogeneous genetic backgrounds among isolates, with an average 3.8 alleles per marker. Isolates identical 15-loci different locations, suggesting free gene flow across Basin. Allopatric carrying SMNT CMNT have similar backgrounds, although CIET haplotype some exclusive alleles, that likely been introduced into Brazil Asia or Africa. This study provides first evidence Asian allele detailed analysis P. populations, respect haplotypes,
oup.com
paperity.org
oxfordjournals.org参考文章(27)
Peter Smouse, J. M. Quattro, L. Excoffier, Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics. ,vol. 131, pp. 479- 491 ,(1992) , 10.1093/GENETICS/131.2.479
Nicholas PJ Day, Catrin E Moore, Mark C Enright, Anthony R Berendt, John Maynard Smith, Michael F Murphy, Sharon J Peacock, Brian G Spratt, Edward J Feil, None, A link between virulence and ecological abundance in natural populations of Staphylococcus aureus. Science. ,vol. 292, pp. 114- 116 ,(2001) , 10.1126/SCIENCE.1056495
Jane MR Carlton, David A Fidock, Abdoulaye Djimdé, Christopher V Plowe, Thomas E Wellems, Conservation of a novel vacuolar transporter in Plasmodium species and its central role in chloroquine resistance of P. falciparum. Current Opinion in Microbiology. ,vol. 4, pp. 415- 420 ,(2001) , 10.1016/S1369-5274(00)00228-9
John E. Hyde, Mechanisms of resistance of Plasmodium falciparum to antimalarial drugs. Microbes and Infection. ,vol. 4, pp. 165- 174 ,(2002) , 10.1016/S1286-4579(01)01524-6
D. Payne, Spread of chloroquine resistance in Plasmodium falciparum Parasitology Today. ,vol. 3, pp. 241- 246 ,(1987) , 10.1016/0169-4758(87)90147-5
Cally Roper, Richard Pearce, Barry Bredenkamp, Jonathan Gumede, Chris Drakeley, Frank Mosha, Daniel Chandramohan, Brian Sharp, Antifolate antimalarial resistance in southeast Africa: a population-based analysis. The Lancet. ,vol. 361, pp. 1174- 1181 ,(2003) , 10.1016/S0140-6736(03)12951-0
Xin-zhuan Su, Michael T Ferdig, Yaming Huang, Chuong Q Huynh, Anna Liu, Jingtao You, John C Wootton, Thomas E Wellems, A Genetic Map and Recombination Parameters of the Human Malaria Parasite Plasmodium falciparum Science. ,vol. 286, pp. 1351- 1353 ,(1999) , 10.1126/SCIENCE.286.5443.1351
Amar Bir Singh Sidhu, Dominik Verdier-Pinard, David A. Fidock, Chloroquine Resistance inPlasmodium falciparumMalaria Parasites Conferred bypfcrtMutations Science. ,vol. 298, pp. 210- 213 ,(2002) , 10.1126/SCIENCE.1074045
Jacques Le Bras, Remy Durand, The mechanisms of resistance to antimalarial drugs in Plasmodium falciparum. Fundamental & Clinical Pharmacology. ,vol. 17, pp. 147- 153 ,(2003) , 10.1046/J.1472-8206.2003.00164.X
Motoo Kimura, A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution. ,vol. 16, pp. 111- 120 ,(1980) , 10.1007/BF01731581