Solid matrix-antibody-antigen complexes induce antigen-specific CD8+ cells that clear a persistent paramyxovirus infection.

摘要: We have previously shown that the adoptive transfer of splenocytes, isolated from mice immunized by infection with paramyxovirus simian virus 5 (SV5), enhance speed clearance SV5 lungs immunodeficient mice; is mediated primarily through CD8+ effector cells and not serum neutralizing antibody (D.F. Young, R.E. Randall, J.A. Hoyle, B.E. Souberbielle, J. Virol. 64:5403-5411, 1990). In this article we demonstrate immunization solid matrix-antibody-antigen (SMAA) complexes also induces are responsible for clearing persistent infections in mice. The demonstration SMAA (an exogenous antigen) can induce class I-restricted cytotoxic T lymphocytes (CTLs) suggests these may be vivo. This premise supported indirectly observation was less efficient inducing CTLs (as measured vitro) than infectious splenocytes were virus. because generally immunogenic virus, since (which contained HN protein SV5) produced higher levels majority experiments, fixed killed suspensions Staphylococcus aureus Cowan strain A used as matrix construction complexes. However, present evidence alum-antibody-antigen S. A-antibody-antigen These results suggest immunological reactivity itself does influence intensity immune response to antigens interest significance vaccine design discussed.