Immunological and cytotoxicological characterization of tuberculin purified protein derivative (PPD) conjugated to single-walled carbon nanotubes.

作者: Majid Zeinali , Mostafa Jammalan , Sussan K. Ardestani , Nader Mosaveri

DOI: 10.1016/J.IMLET.2009.07.012

关键词: MTT assayConjugateImmune systemMolecular biologyReactive oxygen speciesCytotoxic T cellAdjuvantImmunologyChemistryNitric oxideCytotoxicity

摘要: Abstract Tuberculosis (TB) represents one of the leading killers among all infectious disease. Protection against TB depends on activation T-helper type I (Th1) immune response. Carbon nanotubes (CNTs) have attracted considerable attention because their potential applications as new nanovehicle. In current study, tuberculin purified protein derivative (PPD) was conjugated to carboxylated single-walled carbon (SWCNTs). Cytotoxicity SWCNT and SWCNT-PPD conjugate analyzed with MTT assay by reactive oxygen species (ROS) nitric oxide (NO) generation. Male BALB/c mice were immunized BCG, PPD, PPD in complete Freund's adjuvant (CFA). Induction cellular response measuring levels Th1 cytokines (IFN-γ IL-12) Th2 (IL-10 IL-5). Immunization non-conjugated or induced a cytokine while immunization BCG resulted mixed Th1/Th2 contrast, conjugation generated preferentially Th1-type absence cytotoxic effects.

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