Flow Cytometric Analysis of Myeloid Cells in Human Blood, Bronchoalveolar Lavage, and Lung Tissues.

摘要: Clear identification of specific cell populations by flow cytometry is important to understand functional roles. A well-defined panel for myeloid cells in human bronchoalveolar lavage (BAL) and lung tissue currently lacking. The objective this study was develop a cytometry-based BAL tissue. We obtained performed cytometry/sorting on Confocal images were from using antibodies cluster differentiation (CD)206, CD169, E cadherin. defined multicolor that identifies major leukocyte populations. These include macrophage (CD206(+)) subsets other CD206(-) leukocytes. include: (1) three monocyte (CD14(+)) subsets, (2) CD11c(+) dendritic (CD14(-), CD11c(+), HLA-DR(+)), (3) plasmacytoid CD11c(-), HLA-DR(+), CD123(+)), (4) granulocytes (neutrophils, mast cells, eosinophils, basophils). Using tissue, we two pulmonary macrophages: CD169(+) CD169(-) macrophages. In macrophages prominent type. confocal microscopy, located the alveolar space/airway, defining them as contrast, associated with airway/alveolar epithelium, consistent interstitial-associated allows multiple immune types delineates This has implications samples.