Ageing in the mammalian brain

摘要: With a globally ageing population diseases associated with this natural process are becoming major issues worldwide. Research into the of and its concomitant is rapidly expanding; need for new tools models to investigate expanding arena research paramount. The discovery spontaneous mutation in AS rat strain which introduces premature stop gene encoding protein kinase C  (PKC lead development model one such age related disorder, Parkinson‟s disease. Consequently, has been selected changes specific areas brain (the cerebellum, basal ganglia, cerebral cortex brainstem). These regions were because they have previously shown demonstrate (cerebellum, Ganglia), show differences between AS/AGU strains (basal ganglia) or PKC knockout (cerebellum). Brainstem was as it shows little change due no knockouts rats. This study used established qPCR methods measure validated biomarker ageing, CDKN2A transcript p16INK4a) brains these rats determine whether fact genuine accelerated ageing. thesis demonstrates that expression, combination senescence-associated -galactosidase staining, provides clear evidence when compared parent strain. These investigations furthered by an investigation members Sirtuin family proteins. expression Sirtuins indicates there may be increased levels cellular stress, disruption metabolism DNA damage rats, would congruent phenotype present strain. Furthermore, line predictions from MTR trinity regards phenotype. Whilst some senescence metabolic attributable appear element independent mutation.