Hydrogen gas alleviates oxygen toxicity by reducing hydroxyl radical levels in PC12 cells

摘要: Hyperbaric oxygen (HBO) therapy through breathing at the pressure of above 1 atmosphere absolute (ATA) is useful for varieties clinical conditions, especially hypoxic-ischemic diseases. Because generation reactive species (ROS), gas high pressures can cause toxicity in central nervous system, leading to multiple neurological dysfunction, which limits use HBO therapy. Studies have shown that Hydrogen (H2) diminish oxidative stress and effectively reduce active ROS associated with However, effect H2 on generated from remains unclear. In this study, we investigated during using PC12 cells. cells cultured medium were exposed or mixed ATA 5 ATA. Cells viability oxidation products determined. The data showed promoted cell inhibited damage mitochondria membrane, reduced levels lipid peroxidation DNA oxidation, selectively decreased •OH but not disturbing O2•-, H2O2, NO• These results indicated •OH, protected against resulting therapy, had no other ROS. Our supported could be potentially used as an antioxidant