Hydrogen protects auditory hair cells from cisplatin-induced free radicals

摘要: Cisplatin is a widely used chemotherapeutic agent for the treatment of various malignancies. However, its maximum dose often limited by severe ototoxicity. ototoxicity may require production reactive oxygen species (ROS) in inner ear activating enzymes specific to cochlea. Molecular hydrogen was recently established as an antioxidant that selectively reduces ROS, and has been reported protect central nervous system, liver, kidney cochlea from oxidative stress. The purpose this study evaluate potential molecular cochleae against cisplatin. We cultured mouse cochlear explants medium containing concentrations cisplatin examined effects gas dissolved directly into media. Following 48-h incubation, presence intact auditory hair cells assayed phalloidin staining. caused cell loss dose-dependent manner, whereas addition significantly increased numbers remaining cells. Additionally, hydroxyphenyl fluorescein (HPF) staining spiral ganglion showed formation hydroxyl radicals successfully reduced hydrogen-treated cochleae. These data suggest can tissues toxicity, thus providing additional strategy drug-induced damage.