Efficient antitumor immunity derived from maturation of dendritic cells that had phagocytosed apoptotic/necrotic tumor cells.

摘要: Dendritic cells (DCs) that acquired antigen from apoptotic tumor are able to induce major histocompatibility complex (MHC) class I-restricted cytotoxic T lymphocytes and antitumor immunity. In the present study, we investigated efficiency of immunity derived DCs had phagocytosed apoptotic/necrotic BL6-10 melanoma compared with pulsed mTRP2 peptide. Our data showed phagocytosis resulted in maturation up-regulated expression proinflammatory cytokines [interleukin (IL)-1β, IL-6, necrosis factor-α, interferon-γ granulocyte-macrophage colony-stimulating factor], chemokines (MIP-1α, MIP-1β MIP-2), CC chemokine receptor CCR7 cell surface molecules (MHC II, CD11b, CD40 CD86), down-regulated receptors CCR2 CCR5. These mature displayed enhanced migration toward MIP-3β a chemotaxis assay vitro regional lymph nodes an animal model vivo. also vaccination was (i) more strongly stimulate allogeneic T-cell proliferation vitro, (ii) vivo Th1-type immune response leading efficient tumor-specific CD8+ T-cell-mediated (iii) eradicate lung metastases all 6 vaccinated mice peptide, which were reduced (mean number 16 per mouse) but not completely eradicated. Therefore, appear offer new strategies DC cancer vaccines. © 2001 Wiley-Liss, Inc.