Adoptive immunotherapy by avidin-driven cytotoxic T lymphocyte-tumor bridging.
作者： Fabrizio Veglia , Marco Chinol , Angelo Corti , Giovanni Paganelli , Maria Guttinger
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摘要: We have shown previously that T cells, tagged with biotinylated anti-CD3 antibody fragments, can exert avidin-dependent cytolytic activity on suitably tumor cells in vitro . In this study, we demonstrate avidin-driven CTL-tumor bridging in vivo leads to growth inhibition of murine tumors WEHI-164 fibrosarcoma and RMA lymphoma. The biodistribution biotin-tagged 111 In-labeled demonstrated a selective avidin-dependent time-dependent accumulation radioactivity at tumor sites. specificity lymphocyte localization was demonstrated by the concurrent decrease radioactivity in blood all other organs. Furthermore, documented a therapeutic effect adoptively transferred cells, i.e., significant delay at early stages. All experiments included control group mice, which received reagents, except avidin. These avidin-minus mice showed no specific growth, indicating avidin was essential for T-cell
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