Prophylactic anti-tumor immunity against a murine fibrosarcoma triggered by the Salmonella type III secretion system.

摘要: Abstract The potential of an attenuated Salmonella enterica serovar Typhimurium strain as a prophylactic anti-tumor vaccine against the murine fibrosarcoma WEHI 164 was evaluated. Tumor cells were transfected with DNA sequence encoding MHC class I-restricted peptide p60 217–225 from Listeria monocytogenes . BALB/c mice received single orogastric immunization that translocates chimeric protein via its type III secretion system. Mice subsequently challenged subcutaneously -expressing cells. In vivo protection studies revealed 80% these remained free after challenge, whereas all animals non-vaccinated control group did develop tumor growth. further experiments, distribution tetramer-positive -specific effector and memory CD8 T -based application analyzed. Costaining CD62L CD127 predominance p60-specific central in spleens, blood samples majority lymphocytes belonged to cell subsets. This is first report demonstrating bacterial system can be used for heterologous antigen delivery induce cytotoxic responses resulting efficient prevention