Improving storage stability of recombinant organophosphorus hydrolase
作者： A.R. Satvik Iyengar , Rajan K. Tripathy , Priyanka Bajaj , Abhay H. Pande
DOI: 10.1016/J.PEP.2015.01.012
关键词:
摘要: Abstract Organophosphorus hydrolase (OPH) is a ∼38 kDa enzyme encoded by opd gene of Flavobacterium sp. The can hydrolyze and inactivate variety organophosphate (OP)-compounds, including chemical warfare nerve agents. Thus, OPH strong candidate for the development therapeutic intervention against OP-poisoning in humans other animals. It also promising bio-decontaminating agent clean-up OP-contaminated objects areas. For successful commercial application, long-term storage stability purified important. In this study we have cloned expressed recombinant (r-OPH) Escherichia coli effect different excipients on was analyzed. stored either aqueous solution or lyophilized form at 25 °C 60 days presence absence determined monitoring paraoxon-hydrolyzing activity. Our results suggest that, (a) maltose, trehalose, arginine proline were most effective stabilizing when buffer 25 °C, (b) mannose exerted maximum stabilization 60 days. shows that common be used to stabilize order store it long period time under conditions. develop formulation(s) use.
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