Immunization with Mimotopes Prevents Growth of Carcinoembryonic Antigen–Positive Tumors in BALB/c Mice

摘要: Purpose: The carcinoembryonic antigen (CEA) is a glycoprotein that overexpressed in nearly 50% of all human and veterinarian tumors. At present, anti-CEA antibodies are being tested clinical studies as passive immunotherapeutics. This study aims to establish an active immunotherapy for the poorly immunogenic CEA by generating surrogates. Experimental Design: We used monoclonal antibody Col-1 biopanning method generate peptide mimics (mimotopes) epitope. showing highest specificity mimicry was synthesized octameric multiple antigenic mimotope (MAM). Subsequently, immunogenicity selected examined BALB/c mice. assessed antibody-dependent cellular cytotoxicity complement-dependent mediated induced on CEA-expressing HT29 tumor cells. Furthermore, after immunization, mice were transplanted s.c. with Meth-A/CEA Results: When immunized this MAM, they generated specific humoral immune response against CEA. mimotope-induced polyclonal poly-isotypic vitro . when MAM-immunized cells expressing CEA, suppressed growth observed. Conclusion: From our results, we can conclude epitope be translated into mimic. recognize do effectively inhibit CEA-positive Based these finding, suggest mimotopes candidates