Biological effects in vitro of monoclonal antibodies to human epidermal growth factor receptors.

摘要: Four mouse hybridomas secreting monoclonal immunoglobulin G (IgG) antibodies to epidermal growth factor (EGF) receptors of A431 cells were obtained independently from four fusion experiments. Three the antibodies, 528 IgG, 225 and 579 inhibited binding [125I]EGF by at least 95%, they competed with each other for cells. These bound cells, HeLa-S human foreskin fibroblasts dissociation constants in range Kd = 0.6 X 10(-9) 2.5 M. The fourth antibody, 455 lower affinity (Kd 2.0 10(-8) M), it had no effect on either EGF or All immunoprecipitated Triton X-100 extracts membranes, but unable bind three rodent cell lines. In biological assays, none was able mimic EGF. which blocked EGF-enhanced phosphorylation membrane proteins induced fibroblast proliferation. antagonistic also partially reversed inhibition contrast, IgG early delayed cellular responses