Growth effects of epidermal growth factor (EGF) and a monoclonal antibody against the EGF receptor on four glioma cell lines.

摘要: Epidermal growth factor (EGF) has been shown to stimulate DNA synthesis and cell division in normal glia. At least half of malignant human gliomas (MHG) express high levels the EGF receptor (EGFR), which are above those detected brain. The demonstration that antibodies against EGFR inhibit squamous carcinoma line A-431, with large numbers EGFR, vitro vivo raises possibility these agents could be used therapeutically either alone or conjugated other agents. We have measured effects an anti-EGFR monoclonal antibody, 528 (Ab-528), on four well-characterized glioma lines, D-263 MG, D-247 U-343 MGa Cl 2∶6, D-37 2.9×104, 1.5×105, 8.6×105 1.59×106 EGFRs per cell, respectively. significantly increased number MG by 65% 74%, respectively, had no effect decreased 2∶6 39%. was inhibited 19% Ab-528, but Ab-528 MHG lines. all EGF-mediated effects. These studies demonstrate that, although little antiproliferative activity MGH, it successfully competes reduce biological EGF-EGFR binding. Therefore, this antibody potentially target radioisotopes via for diagnosis therapy.