Growth Inhibition of Human Tumor Cells in Athymic Mice by Anti-Epidermal Growth Factor Receptor Monoclonal Antibodies

摘要: Abstract Monoclonal antibodies (MoAbs) were raised against epidermal growth factor (EGF) receptors on a human epidermoid carcinoma cell line, A431. Administration of anti-EGF receptor MoAbs inhibited tumor formation in athymic mice by A431 cells and another T222. When one the same was used therapy Li-7 (a hepatoma) HeLa cervical carcinoma), not affected. The number EGF about 100-fold higher than T222, Li-7, cells, suggesting that may be an important determinant suppressing growth. Three present studies. 528 (immunoglobulin G2a) 225 G1) are capable competing with for binding inhibit proliferation culture. other MoAb, 455 G1), is incapable blocking to its has no effect cultured cells. All three mice, indicating antibody isotype site determinants antiproliferative activity vivo . observation MoAb cytostatic rather cytocidal vitro yet completely prevents , suggests some host animal responses also involved antitumor effect. could provide useful immunotherapeutic agents.