Cytotoxic effects of Ad5CMV-p53 expression in two human nasopharyngeal carcinoma cell lines.

摘要: Nasopharyngeal carcinoma (NPC) is a malignant disease of the head/neck region with 5-year survival level approximately 65%. To explore novel therapeutic strategies in management this disease, potential Ad5CMV-p53-mediated gene transfer to NPC cells was investigated vitro. Two cell lines, CNE-1 and CNE-2Z, were infected either Ad5CMV-p53 or Ad5CMV-beta-galactosidase evaluated for transduction efficiency cytotoxicity. At multiplicity infection 50 plaque-forming units (pfu)/cell, beta-galactosidase expression detected almost 100% treated cells. High levels recombinant p53 protein also observed lines when at pfu/cell. Expression dose time dependent, peak 24 h. A marked increase WAF1/CIP1 after infection. bcl-2 bax minimally detectable baseline; induced no changes Growth be significantly inhibited determined by 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide clonogenic assay. Infection 25 pfu/cell resulted 0.35 11% CNE-2Z cells, respectively. Chromatin condensation DNA fragmentation observed, demonstrating that these undergoing apoptosis. However, GM38 (normal human fibroblasts) subjected identical treatments, they demonstrated lower transgene resistant These data demonstrate efficacy therapy NPC, thus warranting additional investigations strategy.