SLAIN2 links microtubule plus end-tracking proteins and controls microtubule growth in interphase.

摘要: The ends of growing microtubules (MTs) accumulate a set diverse factors known as MT plus end–tracking proteins (+TIPs), which control microtubule dynamics and organization. In this paper, we identify SLAIN2 key component +TIP interaction networks. We showed that the C-terminal part bound to end-binding (EBs), cytoplasmic linker (CLIPs), CLIP-associated characterized in detail with EB1 CLIP-170. Furthermore, found N-terminal interacted ch-TOG, mammalian homologue polymerase XMAP215. Through its multiple interactions, enhanced ch-TOG accumulation at and, consequence, strongly stimulated processive polymerization interphase cells. Depletion or disruption SLAIN2–ch-TOG complex led disorganization radial array. During mitosis, became highly phosphorylated, EBs was inhibited. Our study provides new insights into molecular mechanisms underlying cell cycle–specific regulation organization network.