Epigenetic and molecular signatures of cord blood CD34(+) cells treated with histone deacetylase inhibitors.

摘要: Background and Objectives Epigenetic modifications tightly regulate the gene expression cellular function of haematopoietic stem cells. Histone deacetylase inhibitors (HDACIs) alter profile cord blood (CB) CD34+ cells by controlling genes involved in chromatin modification, thereby influencing self-renewal, maintenance expansion progenitor (HSPCs). Materials Methods The class I II HDACIs, valproic acid scriptaid, were utilized to expand CB-CD34+ ex vivo. The profiling was performed on HSPC using Illumina microarray, GeneGO MetaCore™ Ingenuity pathway analyses. molecular analyses Q-PCR Western blotting. Results Each HDACI treatment created unique epigenetic signatures that governed modification required for functional behaviour established understanding epigenetically regulated HSPCs presence scriptaid VPA revealed different network(s) potential regulators during erythropoiesis. induced transcriptional activation glucocorticoid receptor (GCR) an increase intracellular signalling signal transducers activators transcription (STAT) stress Canonical Wnt many remodellers significantly influenced so as establish regulation HSPC. Conclusion Treatment with Individual HDACIs has demonstrated CB-HSPC. This study identifies key gives insights into clinically important processes lineage development transplantation purposes.