Synthesis, characterization, and the antifungal activity of chitosan derivatives containing urea groups.

摘要: As an abundant and renewable polysaccharide, chitosan has been drawing broad attention due to its natural properties. For the further utilization of chitosan, chemical modification can be applied in improving water solubility bioactivities. In this study, four derivatives were synthesized by reaction chloracetyl derivative with quaternary ammonium salt (CTCS) urea groups bearing 4-amino-pyridine, including BCTCS, 2CBCTCS, 3CBCTCS, 4CBCTCS. The structure characteristics products established based on FT-IR spectroscopy, 1H NMR, elemental analysis. Their antifungal activities against Fusarium oxysporum f. sp. niveum, Phomopsis asparagus, cucumebrium Owen, Botrytis cinerea estimated hyphal measurement vitro. Generally, inhibition ratio most was higher than 80% at 1.0mg/mL. inhibitory activity decreased roughly order: 4CBCTCS>3CBCTCS>2CBCTCS>BCTCS>CTCS>chitosan, resulted from different degrees substitution effectively active groups-urea groups. Meanwhile, effects beneficial soil microbes, Bacillus cereus, subtilis, Sinorhizobium saheli LMG7837, these evaluated disk diffusion method. results showed that substances do not inhibit growth bacteria some them could employed as green biomaterials.